Impedimetrický biosensor pro profilování onkomarkerů prostaty založený na magnetických jádrech se zlatými nanoobaly

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Popis významného výsledu je pouze v anglické verzi.

In this paper, several advances were implemented for glycoprofiling of prostate specific antigen (PSA), which can be applied for better prostate cancer (PCa) diagnostics in the future:

  1. application of Au nanoshells with a magnetic core (MP@silica@Au);
  2.  use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobilization of zwitterionic molecules via diazonium salt grafting;
  3.  a double anti-fouling strategy with the integration of zwitterionic molecules on Au surface and on MP@silica@Au particles was implemented to resist non-specific protein binding;
  4. application of anti-PSA antibody modified Au nanoshells with a magnetic core for the enrichment of PSA from a complex matrix of a human serum;
  5. direct incubation of anti-PSA modified MP@silica@Au with affinity bound PSA to the lectin modified electrode surface.

The electrochemical impedance spectroscopy (EIS) signal was enhanced 43 times integrating Au nanoshells with a magnetic core compared to the biosensor without them. This proof-of-concept study shows that the biosensor could detect PSA down to 1.2 fmol/L and at the same time to glycoprofile such low PSA concentration using a lectin patterned biosensor device. The biosensor offers a recovery index of 108%, when the serum sample was spiked with a physiological concentration of PSA (3.5 ng mL-1).

Au nanoshells
Popis
Scheme and transmission electron micrographs of complex magnetic nanoparticles based on Mn-Zn ferrite cores coated with silica and then encapsulated into gold nanoshell (MP@silica@Au). These particles were subsequently modified by deposition of diazonium-derived zwitterionic self-assembled monolayer and finally by immobilization of anti-fPSA for selective PSA enrichment.

Article: 

T. Bertok, L. Lorencova, S. Hroncekova, V. Gajdosova, E. Jane, M. Hires, P. Kasak, O. Kaman, R. Sokol, V. Bella, A. Andicsova Eckstein, J. Mosnacek, A. Vikartovska, and J.Tkac, Advanced impedimetric biosensor configuration and assay protocol for glycoprofiling of a prostate oncomarker using Au nanoshells with a magnetic core, Biosensors and Bioelectronics, Volume 131, 15 April 2019, Pages 24-29.