Physical stimuli from the cellular microenvironment play a crucial role in regulating key cellular functions such as proliferation, migration, and malignant transformation. Tumor cells, in particular, encounter varying mechanical stimuli that can induce significant metabolic changes. However, the specifics of how tumor cells adapt their metabolism to these mechanical cues remain unclear.
Our research focuses on how liver tumor cells adapt their metabolic activity in response to physical stimuli. We aim to elucidate the effects of 3D culturing on lipid metabolism in liver cancer cells. We hypothesize that mechanical disruption and alterations of the nuclear periphery/lamina and key organelles (e.g., lysosomes, mitochondria, and endoplasmic reticulum) due to the progressive accumulation of lipids will impact genome regulation, ultimately altering cell functionality and promoting tumor expansion.
By investigating these mechanisms, we seek to understand how physical stimuli influence metabolic pathways and contribute to tumor progression. Our research could provide insights into novel therapeutic targets and strategies for combating liver cancer by disrupting the mechanical and metabolic adaptations of tumor cells.
