Scalable production of tissue-like vascularized liver organoids from human PSCs

Authors

S. P. Harrison, R. Siller, Y. Tanaka, M.E. Chollet, M.E. de la Morena-Barrio, Y. Xiang, B. Patterson, E. Andersen, C. Bravo-Pérez, H. Kempf, K. S. Åsrud, O. Lunov, A. Dejneka, M. C. Mowinckel, B. Stavik, P. M. Sandset, E. Melum, S. Baumgarten, F. Bonanini, D. Kurek, S. Mathapati, R. Almaas, K. Sharma, S. R. Wilson, F. S. Skottvoll, I. C. Boger, I. L. Bogen, T. A. Nyman, J. J. Wu, A. Bezrouk, D. Cizkova, J. Corral, J. Mokry, R. Zweigerdt, I. H. Park, G. J. Sullivan. 

Laboratory / Department
Department of Optical and Biophysical Systems
Citation
Exp. Mol. Med. 2023
DOI
10.1038/s12276-023-01074-1
Year
2023

This research presented an innovative approach to the creation of liver organoids (i.e. reduced and simplified versions of the liver), which is significantly cheaper and easier than the existing methods of their production. It does not require intercellular mass for organoid growth, and uses small molecules to mimic embryonic liver development. The result is the production of organoids with a high degree of similarity to the liver, carrying its key functions such as drug metabolism, serum protein production, urea synthesis and coagulation factor production.

 

Schematic overview of liver organoid differentiation from stem cells. Created with BioRender.com.
Schematic overview of liver organoid differentiation from stem cells. Created with BioRender.com.