A novel electrochemical DNA biosensor using a nanocrystalline boron-doped diamond electrode (B-NCDE) is presented. Surface characterization (SEM, Raman, LSV) confirmed successful O-termination (O-B-NCDE). Low-molecular-weight double-stranded DNA was electrostatically adsorbed onto O-B-NCDE, optimizing deposition potential (0.3V), time (3 min), and concentration (10 mg/mL). LSV was used for dsDNA oxidation studies. The biosensor successfully detected intercalation of five compounds (thioridazine, trimipramine, levomepromazine, imipramine, prochlorperazine) within dsDNA, even differentiating phenothiazines from tricyclic antidepressants. The developed simple bioanalytical protocol is highly promising for the determination of the nature of the interaction between DNA and various drug representatives with the time of analysis slightly below the acceptable 10 min, fast &cheap detection of pesticides, etc. (in contrast to HPLC measurements). This study demonstrates the B-NCDE's novel bioanalytical potential and attracted the attention of the electrochemically-oriented research community.
