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Optical gold elements coated with diamond layers with flat, nanocolumnar, and nanoporous morphologies. Fabricated diamond coated optical elements were successfully used for the grazing angle reflectance FTIR characterization of fetal bovin serum (FBS) proteins adsorbed on the O-terminated diamond surface. In spite of the different processing and structuring, all types of diamond layers showed good stability, uniformity, and optical properties, including good reflectivity of the gold mirrors.